The Italian would like to select an entirely different team to the one that drew 1-1 with Arsenal in the Barclays Premier League. City travel down to London on Saturday for another league game against in-form Fulham and, with the champions stuttering to three draws from their first five games, that is very much Mancini's focus.
The Italian said: "We can't play with the same players. We need to recover because afterwards we have another hard game in London. We will change all the players if it's possible. We are unlucky with the draw because we always get difficult games."
The League Cup is the one domestic trophy Mancini has not won since taking over as City boss in 2009, with his best run coming last season when they lost to Liverpool in the semi-finals.
The Italian added: "We want to win again the championship, that's our target. If we also win the Capital One Cup, then okay, but the target should be the championship again."
Mancini will include youngsters Denis Suarez, Abdul Razaq and Luca Scapuzzi in the squad for Tuesday's game while Carlos Tevez and Mario Balotelli are likely to lead the line after only being used as substitutes against Arsenal.
But City will be without four players after James Milner and Maicon joined Samir Nasri and Micah Richards on the sidelines.
Maicon took a kick to his leg against Real Madrid and is unlikely to be out for long but Milner was due to see a specialist about a toe problem.
Mancini admits he does not know what the prognosis will be, saying: "Milner has a problem in his toe. He has pain. He will see a specialist and after that we'll decide what to do."
Source: PA
Source: PA